The impact of swimming training on physiological and psychological indicators in individuals of different age groups: a cross-sectional study

Swimming training is a kind of aquatic exercise that has been shown to be beneficial to physical fitness at any age. This study aimed to determine how mental and physical aspects of swimming affect aerobic and anaerobic capacity describes the physiologicaland psychological effects of swimming training at different ages. This studyexamines how swimming impacts youthful and elderly cardio-respiratory fitness, muscular strength, flexibility, and body composition. A correlation and stepwise regression analysis was performed on the information gathered from a RESTQ-76 sport questionnaire, aHeart Rate Variability (HRV) test, and the efforts of swimmers to improve their aerobic and anaerobic swimming performance. We also investigate the benefits that swimming has on lowering stress, boosting self-esteem, and improving mental health for people of varying ages. We useSPSS version 17.0 was used for the statistical analysis. An examination of the relevant research reveals that swimmers of all ages enjoy significant advantages as a result of their training, including enhancements to their physical and mental health. In that case, they can be able to tailor swimming programs to match the changing physiological and psychological needs of their swimmers. Findings from this study add to a growing body of evidence that open-water swimming is perceived to have positive effects on participants' mental and physical health regardless of age and can be used to inform co-created policy formation to advance outdoor recreation possibilities that are safer, healthier, and more sustainable about this expanding outside activity (AU)

Kinematic and electromyography characteristics of performing butterfly stroke with different swimming speeds in flow environment.

Objective: To investigate effect of flow speeds on the upper limb muscular activity of butterfly swimmers training in a flow environment. A comparison of kinematic characteristics and muscular activity of upper limbs were made when the swimmers training with different flow speeds in a swimming flume. The purpose was to provide a basis for scientifically formulating special swimming training advice for athletes' training in flow environment. Methods: Ten youth female butterfly swimmers participated in the study with the speed of 70%, 80%, and 90% level of their max speeds. A stroke cycle was divided into four phases (entry, pull, push, and recovery). The kinematic parameters of upper limbs (stroke rate, stroke length, duration of each phase in a stroke cycle) and muscular activity (onset timing, integrated electromyography (iEMG), contribution ratio) of four muscles (Biceps brachii (BB), Triceps brachii (TB), Pectoralis major (PM), and Latissimus dorsi (LD)) were collected and analyzed in different stroke phases. Results: There was no significant difference between stroke rate and stroke length with different flow speeds. There were significant differences among the duration of the four stroke phases. The entry phase had the longest duration, the pull phase had the shortest duration, the push phase was longer than the recovery phase, and the recovery phase was shorter than the entry phase. The BB and PM were activated significantly earlier at 90% of target speed than at 80% of target speed, while the TB was activated significantly later than other two speeds. The muscular contribution ratio of the PM was highest in the pull phase and lowest in the pushing phase. The muscular contribution ratio of the BB was significantly lower in the pushing phase than in other three stroke phases. The muscular contribution of the TB was significantly higher in the recovery phase than in other three stroke phases. The muscular contribution ratio of the LD was highest in the pushing phase, and it was significantly higher in pushing phase and recovery phase than in pull phase. Conclusions: (1) When butterfly athletes training with 70%, 80% and 90% of their max speed in a flow environment, it didn't make significant differences between the kinematic or muscle activation characteristics of the upper limbs movement except the muscle onset timing. (2) Stroke phase was the main factor of the duration and the muscle contribution ratio during butterfly arm stroke for young athletes.

The effects of inspiratory muscle training on swimming performance: A study on the cohort of swimming specialization students.

PURPOSE: Current evidence posits a strong correlation between respiratory muscle function and swimming performance. Despite this, few studies have explored the integration of inspiratory muscle training (IMT) into standard swimming training regimens, which remains an unexplored avenue for improving performance in swimmers. This study aims to evaluate the potential advantages of IMT for enhancing respiratory function and swimming performance and determine whether such training could induce beneficial physiological adaptations. METHODS: We designed and conducted a randomized controlled trial involving 43 swimming specialization students aged 18-25 years. Participants were randomly allocated to two groups: a control group, which followed regular swimming training, and an experimental group, which complemented the standard training with IMT. The intervention lasted for six weeks. Key outcomes measured included swimming performance metrics (time of 50 m freestyle, number of breaths in 50 m freestyle, distance before first breath in freestyle, time of 100 m freestyle) and various inspiratory muscle function parameters such as vital capacity (VC), maximum inspiratory pressure (MIP), maximum inspiratory flow (MIF), and maximum inspiratory capacity (MIC). We also assessed certain biochemical parameters, including hemoglobin, creatine kinase (CK), blood urea nitrogen (BUN), testosterone, and cortisol concentrations. RESULTS: Following the training period, the experimental group exhibited significant improvements in swimming performance and respiratory function parameters. We also noted an increase in hemoglobin levels and a reduction in testosterone concentrations in this group, suggesting beneficial physiological adaptations in response to the combined IMT and swimming training. CONCLUSION: Our findings underline the potential of IMT as a supplementary training modality for enhancing respiratory function and improving swimming performance. The changes in biochemical parameters suggest physiological adaptations that might contribute to these observed improvements. This study opens the door for future research on the benefits of integrating IMT into training regimens for competitive swimmers. Further investigation is warranted to fully elucidate the mechanisms behind the observed benefits and to validate these findings in a larger cohort and other athlete populations.

Swimming training and herbal nanoformulations as natural remedies to improve sensory-motor impairment in rat midbrain tumor models: system biology, behavioral test, and experimental validation.

Motor impairment worsens health-related quality of life in patients with primary and metastatic midbrain tumors. Here, 56-male-Wistar rats were divided into eight groups: Normal group, Midbrain Tomur Model group, Model + Exe group, Model + Lipo, Model + Extract, Model + Lipo-Extract, Model + Extract-Exe, Model + Lipo-Extract + Exe. According to the aim, mid-brain tumor models were conducted by injections of the C6 glioma cell line (5 × 105 cell suspension) and stereotaxic techniques in the substantia nigra area. Furthermore, consumption of nanoformulation of herbals extract (100 mg/kg/day), crude extract (100 mg/kg/day), and swimming training (30 min, 3 days/week) as interventional protocols were performed for 6 weeks. In addition, we evaluated the effect of polyherbal nanoliposomes containing four plant extracts and swimming training on the GABArα1/TRKB/DRD2/DRD1a/TH network in the substantia nigra of the midbrain tumor rat model. Data emphasized that DRD2 might be a druggable protein with the network's highest significance cut-point effect that could modulate sensory-motor impairment. Furthermore, we found Quercetin, Ginsenosides, Curcumin, and Rutin, as bioactive compounds present in Ginseng, Matthiola incana, Turmeric, and Green-Tea extracts, could bind over the DRD2 protein with approved binding affinity scores. Based on our data, swimming training, and nanoliposome-enriched combined supplements could consider effective complementary medicine for motor impairment recovery induced by the midbrain tumor in the substantia nigra area. Hence, regular swimming training and natural medicines rich in polyphenolic bioactive components and antioxidative effects could modify and improve the dopamine receptors' function. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03574-3.

The Beneficial Effect of Swimming Training Associated with Quercetin Administration on the Endothelial Nitric Oxide-Dependent Relaxation in the Aorta of Rats with Experimentally Induced Type 1 Diabetes Mellitus.

Type 1 diabetes mellitus is related to the vascular oxidative and nitrosative stress, the trigger for atherosclerosis and cardiovascular complications. The effects of moderate swimming training associated with quercetin oral administration were evaluated in aorta of rats with experimentally induced type 1 diabetes mellitus (T1DM), by analysing the nitric oxide-endothelial dependent relaxation (NO-EDR). T1DM rats received daily quercetin 30 mg/kg and followed the protocol of 5-weeks swimming exercise (30 min/day; 5 days/week). Aorta relaxation to acetylcholine (Ach) and sodium nitroprusside (SNP) were measured at the end of the experiment. Ach-induced endothelial dependent relaxation was significantly decreased in phenylephrine (PE) pre-contracted aorta of diabetic rats. Swimming exercise with quercetin administration preserved Ach-induced EDR but did not have any impact on SNP-induced endothelium-independent relaxation in the diabetic aorta. These findings suggest that quercetin administration associated with moderate swimming exercise could improve the endothelial NO-dependent relaxation in the aorta of rats with experimentally induced type 1 diabetes mellitus, showing that this therapeutical combination may improve and even prevent the vascular complications that occur in diabetic patients.

Swimming training reduced inflammation and apoptotic changes in pulmonary tissue in type 1 diabetic mice.

Background: Despite the vulnerability of pulmonary tissue to diabetic conditions, there are few reports related to the detrimental effects of hyperglycemia and therapeutic modalities on lung parenchyma. Here, the apoptotic changes were monitored in the diabetic pulmonary tissue of mice (DM1) subjected to a four‒week swimming plan. Methods: The mice were randomly allocated into Control; Control + Swimming (S); Diabetic group (D); and Diabetic + Swimming (D + S) groups (each in 8 mice). In the D and D + S groups, mice received intraperitoneally 50 mg/kg of streptozotocin (STZ). After 14 days, swimming exercise was done for four weeks. The expression of il-1ß, bcl-2, bax, and caspase-3 was investigated using real-time PCR analysis. A histological examination was performed using H&E staining. Results: DM1 significantly upregulated il-1ß, bax, and caspase-3, and down-regulated bcl-2 compared to the non-diabetic mice (p < 0.05). We noted that swimming exercises reversed the expression pattern of all genes in the diabetic mice and closed to basal levels (p < 0.05). Data indicated that swimming exercise could diminish emphysematous changes, and interstitial pneumonitis induced by STZ. Along with these changes, swimming exercise had protective effects to reduce the thickness of the inter-alveolar septum and mean alveolar area in diabetic mice. Conclusion: These data demonstrated that swimming exercises could decrease DM1-related pathologies in mouse lungs by regulating apoptosis and inflammatory response.

Integration of Metabolomics and Transcriptomics to Reveal the Metabolic Characteristics of Exercise-Improved Bone Mass.

(1) Background: Exercise is effective in promoting and maintaining bone mass. The aim of this study was to detect the exercise-induced metabolic changes in bone tissue of zebrafish. (2) Methods: Thirty-eight zebrafish (Danio rerio, six months old) were analyzed. The exercise group (n = 19) received 8 weeks of counter-current swimming training. The control group (n = 19) was not subjected to exercise. Mineralization was quantified, and alkaline phosphatase (Alp) and anti-tartrate acid phosphatase (Trap) activities were estimated (n = 12). The metabolomics (n = 12) and transcriptomics (n = 14) data of bone tissue were used for the integration analyses. (3) Results: The results showed that the exercise training improved the bone mineralization of zebrafish, e.g., the exercise group (5.74 × 104 ± 7.63 × 103) had a higher mean optical density than the control group (5.26 × 104 ± 8.56 × 103, p = 0.046) for the caudal vertebrae. The amount of mineralized matrix in scales of the exercised zebrafish was also higher (0.156 ± 0.012 vs. 0.102 ± 0.003, p = 0.005). Both histological staining and biochemical analysis revealed increased Alp activity (0.81 ± 0.26 vs. 0.76 ± 0.01, p = 0.002) and decreased Trap activity (1.34 ± 0.01 vs. 1.36 ± 0.01, p = 0.005) in the exercise group. A total of 103 different metabolites (DMs, VIP ≥ 1, fold change (FC) ≥ 1.20 or ≤0.83, p < 0.050) were identified. Alanine, aspartate and glutamate metabolism, ß-alanine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis were the significantly enriched metabolic pathways (p < 0.050). A total of 35 genes (q ≤ 0.050 (BH), |Log2FC| ≥ 0.5) were coenriched with the 103 DMs in the four identified pathways. Protein-protein interaction network analysis of the 35 genes showed that entpd3, entpd1, and cmpk2 were the core genes. (4) Conclusions: The results of this study suggest that alanine, aspartate and glutamate metabolism, ß-alanine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis contributed to exercise-induced improvements in bone mass.

Dietary Patterns of Competitive Swimmers with Moderate-to-Severe Cerebral Palsy: A 3-Year Longitudinal Evaluation.

AIM: To evaluate the longitudinal dietary patterns of three adolescents with moderate-to-severe cerebral palsy (CP) participating in a performance-focused swimming training intervention. METHOD: Participants were three previously inactive adolescents with CP (15-16 years, GMFCS IV) who had recently (<6 months) enrolled in a swimming training program. Diet quality from diet histories was calculated at 10-time points over 3.25 years using the Dietary Guidelines Index for Children and Adolescents (DGI-CA) and the Healthy Eating Index for Australian Adults (HEIFA-2013). A food group analysis was compared to the Australian Guide to Healthy Eating recommendations. Trends were considered in the context of dietary advice given and the training load. RESULTS: Longitudinal diet quality scores were consistent and ranged from 40 to 76 (DGI-CA) and 33 to 79 (HEIFA-2013). Food group intake remained stable; participants rarely met the recommendations for fruit, vegetables, dairy, grain, and meat but frequently achieved discretionary serves. CONCLUSIONS: Participants with moderate-to-severe CP who were enrolled in a performance-focused swimming training intervention and were monitored frequently maintained diet quality throughout a period where it conventionally declined. Scores were higher than the general population and were maintained irrespective of the training load. Participants frequently met food group recommendations for discretionary foods and were comparable to the general population for other food groups.

Swimming training combined with fecal microbial transplantation protects motor functions in rats with spinal cord injury by improving the intestinal system.

Spinal cord injury (SCI) leads to severe intestinal dysfunction and decreased motility. There is an interaction between the intestine and the nervous system, intestinal intervention through microbial regulation and exercise is a potential treatment option for spinal cord injury. We investigated the effects of swimming rehabilitation training combined with fecal microbial transplantation on intestinal as well as neurological functions in rats with spinal cord injuries, and explored the potential mechanisms. The animals were randomly divided into five groups: sham-operated control group (Sham), spinal cord injury only group (SCI), swimming training group (Swimming), fecal microbial transplantation group (FMT) and combined interventions group (Combined). Behavioral assessments, pathological and immunological analyses were performed after the interventions. Compared to rats in the spinal cord injury group, rats subjected to swimming training, fecal microbial transplantation and combined interventions group exhibited improved intestinal transit, barrier functions, motility, and motor conduction pathway conductivity(P < 0.05). The combined interventions group had better outcomes(P < 0.01). In addition, combined interventions significantly suppressed inflammatory factor levels (P < 0.05) in the colon and spinal cords and significantly protected forefoot motor neurons (NeuN) in the spinal cord injury area, inhibiting astrocyte activation and reducing the expressions of the signature glial fibrillary acidic protein (GFAP) and markers of microglia (Iba-1) at the lesion site(P < 0.05). In conclusion, all effects of combined swimming training and fecal microbial transplantation interventions were superior to swimming training or fecal microbial transplantation alone. Swimming training and fecal microbial transplantation interventions have a synergistic effect on the recovery of intestinal function and motility after spinal cord injury. The mechanism of mutual facilitation between gut function and motility may be related to the brain-gut axis interaction.

Neurogranin as an important regulator in swimming training to improve the spatial memory dysfunction of mice with chronic cerebral hypoperfusion.

BACKGROUND: Vascular cognitive impairment caused by chronic cerebral hypoperfusion (CCH) has become a hot issue worldwide. Aerobic exercise positively contributes to the preservation or restoration of cognitive abilities; however, the specific mechanism has remained inconclusive. And recent studies found that neurogranin (Ng) is a potential biomarker for cognitive impairment. This study aims to investigate the underlying role of Ng in swimming training to improve cognitive impairment. METHODS: To test this hypothesis, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system was utilized to construct a strain of Ng conditional knockout (Ng cKO) mice, and bilateral common carotid artery stenosis (BCAS) surgery was performed to prepare the model. In Experiment 1, 2-month-old male and female transgenic mice were divided into a control group (wild-type littermate, n = 9) and a Ng cKO group (n = 9). Then, 2-month-old male and female C57BL/6 mice were divided into a sham group (C57BL/6, n = 12) and a BCAS group (n = 12). In Experiment 2, 2-month-old male and female mice were divided into a sham group (wild-type littermate, n = 12), BCAS group (n = 12), swim group (n = 12), BCAS + Ng cKO group (n = 12), and swim + Ng cKO group (n = 12). Then, 7 days after BCAS, mice were given swimming training for 5 weeks (1 week for adaptation and 4 weeks for training, 5 days a week, 60 min a day). After intervention, laser speckle was used to detect cerebral blood perfusion in the mice, and the T maze and Morris water maze were adopted to test their spatial memory. Furthermore, electrophysiology and Western blotting were conducted to record long-term potential and observe the expressions of Ca2+ pathway-related proteins, respectively. Immunohistochemistry was applied to analyze the expression of relevant markers in neuronal damage, inflammation, and white matter injury. RESULTS: The figures showed that spatial memory impairment was detected in Ng cKO mice, and a sharp decline of cerebral blood flow and an impairment of progressive spatial memory were observed in BCAS mice. Regular swimming training improved the spatial memory impairment of BCAS mice. This was achieved by preventing long-term potential damage and reversing the decline of Ca2+ signal transduction pathway-related proteins. At the same time, the results suggested that swimming also led to improvements in neuronal death, inflammation, and white matter injury induced by CCH. Further study adopted the use of Ng cKO transgenic mice, and the results indicated that the positive effects of swimming training on cognitive impairments, synaptic plasticity, and related pathological changes caused by CCH could be abolished by the knockout of Ng. CONCLUSION: Swimming training can mediate the expression of Ng to enhance hippocampal synaptic plasticity and improve related pathological changes induced by CCH, thereby ameliorating the spatial memory impairment of vascular cognitive impairment.

Vitamin D supplementation combined with aerobic physical exercise restores the cell density in hypothalamic nuclei of rats exposed to monosodium glutamate.

BACKGROUND & AIMS: In view of the increase in the prevalence of obesity and metabolic syndrome in childhood and adolescence, this study proposed the early and combined use of treatments to restore brain areas related to satiety. The vitamin D supplementation, aerobic exercise and the combination of these interventions on the structure of arcuate (ARC) and ventromedial (VMH) nuclei of hypothalamus were investigated in monosodium glutamate (MSG)-treated rats. METHODS: Wistar rats were separated into five groups: Control group (CT); Obese group injected with MSG (OB); Obese group supplemented with vitamin D (OBvd); Obese group submitted to forced swimming training (OBexe) and Obese group treated with vitamin D supplementation and forced swimming training (OBvd + exe). RESULTS: In the OB group, the visceral fat weight was significantly higher, there was a reduction in the number of glial cells in the ARC nucleus and also in the number of neurons in the ARC and VMH nuclei. Aerobic exercise was able to reduce the visceral fat weight in the OBexe group. The combination of treatments used in the OBvd + exe group reversed the loss of neurons and glial cells produced by MSG in the ARC nucleus. All treated groups exhibited a higher number of neurons in VMH nucleus, but an increase in the glial cells were observed only in the OBexe and OBvd + exe groups. CONCLUSIONS: The effectiveness of obesity treatment can be favored through the early and combined use of vitamin D supplementation and aerobic exercise, since these therapies are able to restore brain nuclei involved in the control of food intake.

Investigación del efecto del curso de educación básica de natación en algunos parámetros respiratorios de estudiantes universitarios / Investigation of the effect of basic swimming education course on some respiratory parameters of university students

In this study, the effect of 12-week basic swimming training of sports science students on some respiratory parameters was determined. In the 2nd, 3rd and 4th grades of Gümüşhane University School of Physical Education and Sports, 64 (111) male students who did not take swimming lessons and 47 who took swimming lessons participated voluntarily. The experimental group was given breath work for 12 weeks during the course content and for 10 minutes at the end. Measurements were taken from the students twice before and after the 12-week education. SPSS 25.0 program was used in the analysis of the data collected within the scope of the research. Independent comparison of age, height and body weight measurements of the athletes in the experimental and control groups. Samples T Test was used. In the comparison of time, group, group x time changes of FVC, MVV, VC and FEV1 measurements, Repeated Measures ANOVA analysis was used. Statistical significance level was taken as (p<0.05). As a result of the research, a significant result could not be reached in FVC and FEV1 values between Tests, Groups and Group x time intervals, whereas VC was only between tests. However, statistically significant differences were found between MVV and VC Tests, Groups and Group x Time values. In short, it has been shown that students who take swimming lessons have statistically higher lung volumes than students who do not take the lesson. However, swimming activity can be recommended to increase respiratory efficiency of university students. (AU)

En este estudio, se determinó el efecto del entrenamiento básico de natación de 12 semanas de estudiantes de ciencias del deporte sobre algunos parámetros respiratorios. En los grados segundo, tercero, cuarto de la Escuela de Educación Física y Deportes de la Universidad de Gümüşhane, 64 (111) estudiantes varones que no tomaron clases de natación y 47 que tomaron clases de natación participaron voluntariamente. Al grupo experimental se le dio trabajo de respiración durante 12 semanas durante el contenido del curso y durante 10 minutos al final de la lección. Se tomaron pruebas de los estudiantes dos veces antes y después de la educación de 12 semanas. Se utilizó el programa SPSS 25.0 (Samples-T test) en el análisis de los datos recolectados dentro del alcance de la investigación. Comparación independiente de las medidas de edad, altura y peso corporal de los atletas en los grupos experimental y de control. En la comparación de los cambios de tiempo, grupo, grupo x tiempo de las mediciones de FVC, MVV, VC y FEV1, se utilizó el análisis ANOVA de medidas repetidas. El nivel de significancia estadística se tomó como (p<0,05). Como resultado de la investigación, no se pudo alcanzar un resultado significativo en los valores de FVC y FEV1 entre Pruebas, Grupos y Grupo x intervalos de tiempo, mientras que la VC fue solo entre pruebas. Sin embargo, se encontraron diferencias estadísticamente significativas entre los valores de MVV y VC Tests, Groups y Group x Time. En resumen, se ha demostrado que los estudiantes que toman lecciones de natación tienen volúmenes pulmonares estadísticamente más altos que los estudiantes que no toman la lección. Sin embargo, se puede recomendar la actividad de natación para aumentar la eficiencia respiratoria de los estudiantes universitarios. (AU)

Swimming Training Mitigates Neurological Impairment of Intracerebral Haemorrhage in Mice via the Serine-Threonine Kinase/Glycogen Synthase Kinase 3ß Signalling Pathway.

Swimming training (ST) can mitigate functional disorders in neurological diseases, but the effect and mechanism of ST in improving the neurological function of intracerebral haemorrhage (ICH) have not been reported. Our study aimed to explore the protective effect of early ST on ICH mice and its relationship with the serine-threonine kinase (Akt)/glycogen synthase kinase 3ß (GSK3ß) pathway. Our findings showed that the ICH model mice had poor behavioural manifestations in the Y maze test and open field test compared to the ST group and sham group. The modified neurological severity score was increased in the ICH mice, and 7 days of ST intervention significantly attenuated the neurological deficits. The ratios of myo-inositol/creatine, lactate/creatine and glutamate/creatine were decreased, and the ratios of N-acetylaspartate/creatine and choline/creatine were increased in the ICH mice with ST intervention. ST intervention decreased the expression of Iba1 and GFAP. Seven days of ST significantly increased the expression of p-Akt/Akt compared to that in the ICH mice. Furthermore, the Akt kinase inhibitor GSK690693 exacerbated neurological impairment, increased the expression of Iba1, GFAP and Bax/Bcl-2, and reversed the anti-apoptotic effects and anti-glia activation of ST, which was associated with the inhibition of p-Akt/Akt and p-GSK3ß/GSK3ß expression. These results indicated that the protective role of ST in ICH was mediated via the Akt/GSK3ß pathway. In conclusion, ST displayed neuroprotection by inhibiting apoptosis and glial activation in ICH mice by activating the Akt/GSK3ß signalling pathway.

IL-6 and HSPA1A Gene Polymorphisms May Influence the Levels of the Inflammatory and Oxidative Stress Parameters and Their Response to a Chronic Swimming Training.

The aim of the study was to evaluate whether the most common polymorphisms in the IL-6 and HSP70 genes affect the circulating heat shock protein 70 (HSP70), as well as inflammatory and prooxidant-antioxidant parameters in healthy men undergoing chronic endurance training. The subjects were randomly assigned to a 12-week swimming training (ST group) or control group (CON). Fasting blood samples were collected pre- and post-study period to assessment: superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, serum levels of lipid hydroperoxides (LHs), tumor necrosis factor α (TNFα), and HSP70. Subjects were genotyped for IL-6-174G/C, HSPA1A + 190 G/C and HSPA1B + 1538 A/G single nucleotide polymorphisms (SNPs) by real-time PCR. After a 12-week study period, a decrease in TNFα, HSP70, and GPx was observed in the ST group, but not the CON group. IL-6 SNP affected serum TNFα levels (main effect of genotype). Higher TNFα levels (pre- and post-study period) was observed in CC CON than in other IL-6 genotypes of CON and ST groups. However, a post-training decrease in TNFα was observed in both GG and CC IL-6 genotypes of ST group. In turn, only GG IL-6 genotype of the ST group was related to a post-training decrease in HSP70 (main time and genotype interaction). Moreover, pre- and post-training LHs were lower in GG than GC/CC HSPA1A genotypes of the ST group (main genotype effect). In conclusion, polymorphisms within the IL-6 and HSPA1A genes seem to affect baseline levels of some inflammatory parameters and prooxidant-antioxidant status and/or their changes after chronic swimming training. However, the results should be confirmed in a study with a larger sample size, one that includes individuals with sedentary lifestyles.

Influence of Swimming Training Session on Selected Saliva Components in Youth Swimmers.

Exercise may induce many changes in biochemical parameters of the saliva. Thanks to non-invasive access, saliva can be used as a diagnostic material in physical activity monitoring. The aim of the study was comparison of selected salivary components in swimmers before and after training session. 40 male subjects aged 12-15, out of whom 30 were competitive swimmers and 10 control were involved in the study. Salivary samples were collected from all subjects in the morning, and in the afternoon; from the swimmers, they were also collected before and after the workout. Salivary flow rate-V, pH, total protein-P, alpha-amylase-Amy, salivary peroxidase-SPO, cortisol-C, total antioxidant status-TAS, sialic acid (free-FSA, bound-GSA, total-TSA), calcium-Ca, magnesium-Mg were measured. The swimmers assessed the workout intensity of training session using the RPE Foster's scale. The circadian rhythm pattern of some salivary components and differences after training were found. In swimmers after the morning exercise significant increase of P (0.83 ± 0.27 vs. 1.10 ± 0.58 g/L), Amy (64.91 ± 70.86 vs. 87.07 ± 92.46IU/L), Ca (3.83 ± 1.33 vs. 4.99 ± 2.24 mg/L), Mg (0.52 ± 0.32 vs. 0.73 ± 0.34 mg/L), TAS (0.64 ± 0.27 vs. 0.72 ± 0.26 mmol/L) and decrease V (0.47 ± 0.37 vs. 0.36 ± 0.22 mg/min), C (5.86 ± 5.00 vs. 3.54 ± 5.07 µg/ml) were found. After the afternoon training significant increase of pH (7.13 ± 0.33 vs. 7.27 ± 0.24), Amy (111.53 ± 120.13 vs. 130.91 ± 161.14IU/L), Ca (3.72 ± 1.34 vs. 4.61 ± 1.58 mg/L), Mg (0.48 ± 0.28 vs. 0.60 ± 0.39 mg/L), TSA (5.64 ± 3.78 vs. 6.10 ± 3.08 mg/L), GSA (3.00 ± 3.06 vs. 3.38 ± 2.26 mg/L), and decrease of V (0.63 ± 0.63 vs. 0.49 ± 0.39 ml/min) were noticed. Before training in the morning in the swimmers significantly higher of V (0.47 ± 0.37 vs. 0.26 ± 0.15 mg/min), TAS (0.64 ± 0.27 vs. 0.40 ± 0.16 mmol/L), and lower pH (7.01 ± 0.46 vs. 7.53 ± 0.33), P (0.83 ± 0.27 vs. 1.86 ± 1.28 g/L), Amy (64.91 ± 70.86 vs. 146.56 ± 114.45IU/L) compared to the control were found. In the afternoon in swimmers before training session significantly lower pH (7.13 ± 0.33 vs. 7.53 ± 0.49) and Amy (111.53 ± 120.13 vs. 170.98 ± 107.72IU/L) in comparison to the control were noticed. The RPE scores were negatively correlated with V (rho = -0.500, p = 0.05 and pH (rho = -0.382, p = 0.03) measured after the morning session and after the afternoon training with V (rho = -0.570, p = 0.01) and Ca (rho = -0.401, p = 0.08). The levels of salivary flow rate, alpha amylase, cortisol, calcium, magnesium were associated with swimming training session, and showed circadian variation without a significant effect on their responses to exercise.

Effects of swimming training on myocardial protection in rats.

Swimming is important for promoting and maintaining health, as it can increase the efficiency of the cardiovascular system and decrease the occurrence of cardiovascular diseases. The objective of the present study was to examine whether swimming training could decrease myocardial injury in rats caused by myocardial ischemia/reperfusion (I/R). Sprague-Dawley rats were randomized into four groups, namely the Sham, coronary artery occlusion, swimming training and ischemic preconditioning (IPC) groups. Myocardial I/R was induced in anesthetized male Sprague-Dawley rats by a 40-min occlusion followed by a 3-h reperfusion of the left anterior descending coronary artery. The rats were sacrificed after surgery and their hearts were examined. The results demonstrated that the number of TUNEL-positive nuclei and degree of caspase-3 activation were both significantly increased in the myocardium following myocardial I/R in rats, indicating increased cardiomyocyte apoptosis. On the other hand, swimming training decreased the serum levels of creatine phosphokinase, lactate dehydrogenase and cardiac troponin I, and was associated with reduced histological damage and myocardial infarct size. Furthermore, swimming training also reduced TNF-α levels, caspase-3 activation and enhanced Bcl-2 activation, which decreased the number of apoptotic cells in the myocardium. The findings of the present study showed that swimming training and IPC could similarly decrease myocardial injury following myocardial I/R, and may therefore be used as exercise training to effectively prevent myocardial injury.

The perceived knowledge of the menstruation cycle and adjustment of swimming sets by swimming coaches based on menstrual-related issues.

Background: Menstruation is the recurring discharge of the endometrial lining of the uterus as menstrual blood and tissue. The menstruation cycle affects most adolescent females and, although largely overlooked, affects women participating in sports. Objectives: The aim of this study was to determine whether coaches were aware of their swimmers' menstrual cycles and whether coaches considered this information when adjusting training sets. Methods: Within the case study, a partial mixed-method, sequential dominant status approach was used. Data were collected in the form of questionnaires, focus group discussions, and one-on-one interviews. Coaches' awareness of their female swimmers' menstrual cycles was based more on observation than communication from the swimmer. Results: Coaches explained that training is adjusted based on their observations, but whether this is being done correctly during the menstrual cycle requires more research. Swimmers and coaches alike seem to have minimal knowledge of menstruation, its effects on training, and how to adapt to, or overcome, those effects during training or competition. Conclusion: In future, this knowledge could ensure the longevity of female swimmers in the sport. Understanding whether coaches and swimmers recognise the effect of the menstrual cycle within training and competition provides a more inclusive approach to ensure athlete longevity after puberty. This approach is grounded in creating an understanding between the swimmer and coach about the effect of menstruation during training and competition. It ensures an extended and more successful participation which may also assist in dealing with the 'taboo' surrounding menstruation and the female athlete.

Swimming training combined with chitosan supplementation reduces the development of obesity and oxidative stress in high-fat diet-fed mice.

Obesity is often introduced as one of the metabolic disorders caused by imbalance between energy consumption and metabolisable energy intake. Experts in the field considered obesity as one of the robust risk factors for the lifestyle-associated diseases. The present research examined interventional effects of marine chitosan (CS), swimming training (ST) and combination of CS and ST (CS + ST) in the mice fed with high-fat diets (HFD). In this study, sample size was considered more than three in groups. Forty mice were randomly divided into five groups (n 8 per group) including control group (received the standard diet), HFD group (received high-fat food with 20 % fat), HFD + CS group (treated with high-fat food with 5 % CS), HFD + ST group (treated with HFD and ST) and HFD + CS + ST group (treated with high-fat food with 5 % CS and ST). After 8 weeks, the blood glucose, oxidative stress (OS) and lipid profile were measured. The results showed that CS + ST group has more effects in the control of body weight with the increased concentration of HDL-cholesterol, OS inhibition via enhancing the body antioxidant capacity in comparison with the ST or CS alone in HFD-fed mice. Moreover, lipid profile was improved in CS + ST-treated mice compared with HFD-treated mice, and OS inhibition correlated with the greater activities of the antioxidant enzyme enhances the lipid oxidation, cholesterol and fatty acid homoeostasis. The results suggested that a dietary intervention with a combined ST and CS can be a feasible supplementary for human prevention of obesity.

[Effect of swimming training on the expression of PKC δ/p66Shc protein in mouse myocardium].

Objective: To investigate the effects of different intensity of swimming training on p66Shc protein in mouse myocardium. Methods: Fifty Kunming mice were randomly divided into control group (Group C), weight-bearing swimming group (Group E), weight-bearing swimming + drug group (Group ER), non weight-bearing swimming group (Group P), non weight-bearing swimming + drug group (Group PR), with 10 mice in each group. Group C did not exercise. Groups E, ER, P, and PR received swimming training for 4 weeks. Groups E and ER performed weight-bearing swimming with a 3% body weight, and Group P and Group PR were swimming without weight-bearing, 60 min/d, 6 times/w. Mice in ER and PR groups were injected intraperitoneally with Rottlerin (0.3 mg/kg), a PKCδ inhibitor, before the last two exercises. Groups C, E, and P were injected with the same dose of normal saline. Samples were collected after training finished for 24 hours. The protein expressions of PKCδ, P-PKCδ, P66Shc, P-P66shc and NOX2 were detected by Western blot; PKCδ and P66Shc were detected by immunoprecipitation; malondialdehyde (MDA), reactive oxygen species (ROS) and superoxide dismutase (SOD) in myocardium and serum were analyzed by biochemistry. Results: Compared with Group C, the protein expressions of PKCδ, P-PKCδ, P66Shc, P-P66shc and NOX2 in Group E were increased significantly (P＜ 0.01), the serum and myocardial MDA levels, myocardial ROS were increased significantly (P＜0.05 or P＜0.01), and the myocardial SOD activity was decreased (P＜0.01), the PKCδ, P-PKCδ, P-P66shc and NOX2 in Group P were increased significantly (P＜0.05 or P＜0.01), and the myocardial SOD activity was enhanced (P＜0.05). Compared with Group E, the protein expressionS of PKCδ (P＜0.01), P-PKCδ (P＜0.01), P66Shc (P＜0.05), P-P66shc (P＜0.01), NOX2 (P＜0.05) in Group ER was decreased significantly, the protein expression of P66Shc in Group P was decreased significantly (P＜0.05), the myocardial MDA (P＜0.01) and ROS (P＜0.05) were decreased, and the activity of SOD was enhanced (P＜0.01). Compared with Group P, the protein expressions of PKCδ, P-PKCδ and P-P66shc in Group PR were decreased significantly (P＜0.01), while the expression of NOX2 was increased (P＜0.05). Conclusion: Both swimming training of two intensities promoted the increase of PKCδ protein and its phosphorylation in mouse cardiomyocytes. High-intensity swimming training could significantly enhance the expression and phosphorylation level of p66Shc protein, resulting in the production of ROS and the decrease of antioxidant enzyme activity. Low-intensity swimming training enhanced the phosphorylation of p66Shc, but did not promote its protein expression, resulting in the enhancement of myocardial antioxidant capacity and exercise adaptation.

Swimming training and Plantago psyllium ameliorate cognitive impairment and glucose tolerance in streptozotocin-nicotinamide-induced type 2 diabetic rats.

Brain malfunction is common in diabetic patients. On the other hand, a growing body of research points to the beneficial effect of medicinal plants and exercise training on insulin sensitivity and brain function. Therefore, the aim of the present study was to investigate the effect of co-administration of swimming training and Plantago psyllium (mixed with standard pelleted food at a weight ratio of 5%) on learning and memory impairment and glucose tolerance in type 2 diabetic rats. For this purpose, 10 healthy and 40 rats with type 2 diabetes were randomly allocated to five groups: healthy sedentary control group (Con), sedentary diabetic group (D), diabetic group subjected to swimming training (D + Tr), diabetic group receiving P. psyllium (D + Ps), and diabetic group subjected to swimming training and receiving P. psyllium (D + Ps + Tr). Diabetes was induced by a single intraperitoneal injection of nicotinamide (120 mg/kg) and streptozotocin (65 mg/kg) separately with 15 min intervals. Experimental groups were treated with swimming training and P. psyllium independently and simultaneously for 12 weeks. Lipid profile and food intake were measured and also, glucose tolerance was evaluated by glucose area under the curve (AUCg) using an oral glucose tolerance test. Passive avoidance learning (PAL) and memory were evaluated by shuttle box test and cognitive memory was assessed by novel object recognition (NOR) and elevated plus-maze (EPM) tests. Diabetic rats exhibited a significant increase in food intake, lipid profile, and AUCg compared to healthy rats. Step-through latency in the PAL acquisition trial (STL-a) and retention test (STL-r) were significantly lower in diabetic rats than in the control group. In the diabetic group without treatment, time spent in the dark compartment increased compared to the control group in the shuttle box test. Discrimination index and distance traveled reduced in diabetic rats. On the other hand, swimming training and P. psyllium alleviated food intake, lipid profile, and glucose tolerance in diabetic rats. Also, the STL-a, STL-r, discrimination index, and distance travelled in the D + Ps + Tr group were significantly more than the diabetic group. Results showed that 12 weeks of swimming training and receiving P. psyllium improved memory deficit in streptozotocin-nicotinamide-induced type 2 diabetic rats possibly through hypolipidemic and hypoglycemic effects. These results suggest that the administration of swimming training and P. psyllium simultaneously might be an effective intervention for the treatment of diabetes-induced behavioral deficits.